SRX1506796: MCF-7, MASC-Seq, Replicate 3
1 ILLUMINA (NextSeq 500) run: 70.3M spots, 10.6G bases, 4.4Gb downloads
Design: The forward read (R1) is a 31 nt sequence where the first 18 bp is a barcode (cell ID). This is followed by 9 nt of a semi-randomized UMI sequence used to distinguish duplicated transcripts. The reverse read (R2) is 121 nt used to map and annotate transcripts. The insert size between R1 and R2 is approximately 250 nt.
Submitted by: (KTH)
Study:
High-throughput differential expression profiling of single cells using MASC-seqshow Abstracthide AbstractNovel method using multiplex barcoded in vitro transciption for single cell profiling
Sample:
MCF-7, MASC-Seq, Replicate 3Library:
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: PolyA
Layout: PAIRED
Spot descriptor:
1 forward32 reverse
Runs:
1 run, 70.3M spots, 10.6G bases, 4.4Gb